Remember that some mud is treacherously soft, and do not overlook the danger of being cut off on mudflats by a rising tide.
Dig quadrats along line transects, estimating the vertical level of each quadrat. If possible, use coarse sieves to collect macrobenthos; or if this is impractical, collect macrobenthos by hand-sorting. From each quadrat take a sediment sample, about 500 g, and, if obtainable, an interstitial water sample. Keep the complete collections from each station, separately labelled, for later identification of species and quantitative investigations. At each station note the depth of the unblackened layer and any temperature difference between surface and deeper layers.
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